Recombinant Mouse IgG1 Isotype Control Antibody,InvivoGen,bgal-mab9-1

InvivoGen provides arecombinantanti-β-Gal-mIgG1 monoclonal antibody (mAb)  that was previously extracted from hybridoma. It is now expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. Thiscontrol antibodytargetsE. coli'sβ-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displayslow ADCC(antibody-dependent cell-mediated cytotoxicity) andno CDC(complement-dependent cytotoxicity) effector functions [1]. Thesequenceof Anti-β-Gal-mIgG1 is100% murine(constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].