Recombinant Mouse IgG1e3 Isotype Control Antibody - Fc silent (D265A),InvivoGen,bgal-mab15-1

InvivoGen provides arecombinantanti-β-Gal-mIgG1e3 monoclonal antibody (mAb) that was previously extracted from hybridoma. It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided [1]. Thiscontrol antibodytargetsE. coli'sβ-galactosidase (β-Gal), an irrelevant antigen that is not present in mice. Thesequenceof Anti-β-Gal-mIgG1e3 is100% murine(constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. Within the engineered murine IgG1e3 isotype, a point mutation D265A was introduced leading toa complete lossof unwanted Fc-associatedeffector functions[2]. Altogether, these alterations increase antibody performance and overcome immunogenic events, especially upon repeated mAb injections into mice [3].