SMAD2 (D43B4) Rabbit Monoclonal Antibody (Biotinylated)#12584,Cell Signaling Technology (CST),12584

SMAD2 (D43B4) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total Smad2 protein. This antibody does not cross-react with Smad3 protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Platform ID

BAB456192860

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameSMAD2 (D43B4) Rabbit Monoclonal Antibody (Biotinylated)#12584
Cat. No.12584
Accession NumberQ62432
Gene ID (Entrez)62432, 17126
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
Molecular Weight60
ImmunogenIgG
FormulationBiotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.Optimal dilutions/working concentrations should be determined by the end user. Pleasecontact usif you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
StorageSupplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).Heldin, C.H. et al. (1997)Nature390, 465-71.Attisano, L. and Wrana, J.L. (1998)Curr Opin Cell Biol10, 188-94.Derynck, R. et al. (1998)Cell95, 737-40.Massagué, J. (1998)Annu Rev Biochem67, 753-91.Whitman, M. (1998)Genes Dev12, 2445-62.Wrana, J.L. (2000)Sci STKE2000, re1.Attisano, L. and Wrana, J.L. (2002)Science296, 1646-7.Moustakas, A. et al. (2001)J Cell Sci114, 4359-69.Alternate Names7120426M23Rik; Mad; MAD homolog 2; MAD homolog 2 (Drosophila); Mad-related protein 2; Madh; Madh2; Madr2; mMad2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; SMAD 2; SMAD family member 2; Smad-2; Smad2

Synonyms

7120426M23Rik; Mad; MAD homolog 2; MAD homolog 2 (Drosophila); Mad-related protein 2; Madh; Madh2; Madr2; mMad2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; SMAD 2; SMAD family member 2; Smad-2; Smad2

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