SSH1 (E1K3W) Rabbit Monoclonal Antibody#13578,Cell Signaling Technology (CST),13578

SSH1 (E1K3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total SSH1 protein. Based on the absence of sequence homology, this antibody is not expected to recognize SSH2 or SSH3.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:100

Platform ID

BAB383805094

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameSSH1 (E1K3W) Rabbit Monoclonal Antibody#13578
Cat. No.13578
Accession NumberQ8WYL5
Gene ID (Entrez)54434
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:100
Molecular Weight140
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Cofilin is an evolutionarily conserved, actin-binding protein that severs actin filaments during processes that rely on actin filament dynamics, including cytokinesis, cell migration, invasion, and neuronal development. Actin severing and filament depolymerization are regulated through the controlled cycling of cofilin between the phosphorylated and dephosphorylated forms (1). The kinases LIMK and TESK inactivate cofilin by phosphorylating it at Ser3 (2,3). The slingshot homologs (SSH1, SSH2 and SSH3) and chronophin/PDXP phosphatases remove phosphate from cofilin at Ser3, enabling cofilin binding to actin and filament depolymerization (3). LIMK and SSH1 regulate cofilin activity downstream of neuregulin signaling in Schwann cells (4).Slingshot homolog 1 (SSH1) can also dephosphorylate LIMK kinases, suppressing LIMK phosphorylation of cofilin (5). In addition, SSH1 modulates actin dynamics by stabilizing F-actin and promoting actin bundling independent of its cofilin phosphatase activity (6). SSH1 activity is regulated by phosphorylation and protein-protein interaction through various signaling pathways (1). Binding of SSH1 to F-actin stimulates its cofilin phosphatase activity (7).Mizuno, K. (2013)Cell Signal25, 457-69.Toshima, J. et al. (2001)J Biol Chem276, 31449-58.Huang, T.Y. et al. (2006)Curr Opin Cell Biol18, 26-31.Sparrow, N. et al. (2012)J Neurosci32, 5284-97.Soosairajah, J. et al. (2005)EMBO J24, 473-86.Kurita, S. et al. (2007)Genes Cells12, 663-76.Kurita, S. et al. (2008)J Biol Chem283, 32542-52.Alternate NamesFLJ21928; FLJ38102; hSSH-1L; KIAA1298; Protein phosphatase Slingshot homolog 1; slingshot 1; slingshot homolog 1; slingshot homolog 1 (Drosophila); slingshot protein phosphatase 1; SSH-1L; SSH-like protein 1; SSH1; SSH1L

Synonyms

FLJ21928; FLJ38102; hSSH-1L; KIAA1298; Protein phosphatase Slingshot homolog 1; slingshot 1; slingshot homolog 1; slingshot homolog 1 (Drosophila); slingshot protein phosphatase 1; SSH-1L; SSH-like protein 1; SSH1; SSH1L

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