STING (E9X7F) Rabbit Monoclonal Antibody (SignalFlexTMmFluorTMViolet 510 Conjugate)#57837,Cell Signaling Technology (CST),57837

STING (E9X7F) Rabbit mAb (SignalFlexTM mFluorTM Violet 510 Conjugate) recognizes endogenous levels of total STING protein. STING (E9X7F) Rabbit mAb (SignalFlexTM mFluorTM Violet 510 Conjugate) lacks sensitivity in some low-expressing mouse cell lines by immunofluorescence. This lack of sensitivity has not been observed in human cell lines.

Host

Rabbit

Reactivity

Human, Mouse

Conjugate

SignalFlexTMmFluorTMViolet 510 Conjugate

Platform ID

BAB019023951

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameSTING (E9X7F) Rabbit Monoclonal Antibody (SignalFlexTMmFluorTMViolet 510 Conjugate)#57837
Cat. No.57837
Accession NumberQ86WV6, 340061
Gene ID (Entrez)340061
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse
ConjugationSignalFlexTMmFluorTMViolet 510 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7,8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).Ishikawa, H. and Barber, G.N. (2008)Nature455, 674-8.Zhong, B. et al. (2008)Immunity29, 538-50.Sun, L. et al. (2013)Science339, 786-91.Wu, J. et al. (2013)Science339, 826-30.Zhang, Z. et al. (2011)Nat Immunol12, 959-65.Unterholzner, L. et al. (2010)Nat Immunol11, 997-1004.Ishikawa, H. et al. (2009)Nature461, 788-92.Konno, H. et al. (2013)Cell155, 688-98.Alternate Namesendoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; hSTING; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; NET23; SAVI; Stimulator of interferon genes protein; stimulator of interferon protein; stimulator of interferon response cGAMP interactor 1; STING; STING-beta; STING1; TM173; TMEM173; Transmembrane protein 173

Synonyms

endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; hSTING; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; NET23; SAVI; Stimulator of interferon genes protein; stimulator of interferon protein; stimulator of interferon response cGAMP interactor 1; STING; STING-beta; STING1; TM173; TMEM173; Transmembrane protein 173

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