Spark NIR™ 685 anti-human CD62L Antibody, CD62L, DREG-56,BioLegend,304862

Additional reported applications (for the relevant formats) include: Western blotting2,3,9andin vitroblocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).

Host

Mouse

Reactivity

Human

Application

FC -Quality tested

Platform ID

BAB281787879

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameSpark NIR™ 685 anti-human CD62L Antibody, CD62L, DREG-56
Cat. No.304862
HostMouse
RRIDAB_2860808 (BioLegend Cat. No. 304861)AB_2860809 (BioLegend Cat. No. 304862)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberDREG-56
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD62L
ImmunogenConcentrated supernatant from PMA-activated human peripheral blood leukocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

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