Spark NIR™ 685 anti-mouse CD80 Antibody, CD80, 16-10A1,BioLegend,104761

Additional reported applications (for the relevant formats) include: immunoprecipitation2,in vitroandin vivoblocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Host

Armenian Hamster

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB854325573

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameSpark NIR™ 685 anti-mouse CD80 Antibody, CD80, 16-10A1
Cat. No.104761
HostArmenian Hamster
RRIDAB_2924443 (BioLegend Cat. No. 104761)AB_2924443 (BioLegend Cat. No. 104762)
IsotypeArmenian Hamster IgG
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number16-10A1
Concentration0.5 mg/mL
TargetCD80
ImmunogenCHO cell line transfected with mouse B7 (CD80)
PurityThe antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

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