Spark NIR™ 685 anti-mouse Ly-6G Antibody, Ly-6G, 1A8,BioLegend,127666

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.Additional reported applications (for the relevant formats) include: immunohistochemistry9of frozen sections10and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended forin vivostudies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB169130716

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameSpark NIR™ 685 anti-mouse Ly-6G Antibody, Ly-6G, 1A8
Cat. No.127666
HostRat
RRIDAB_2876454 (BioLegend Cat. No. 127665)AB_2876454 (BioLegend Cat. No. 127666)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number1A8
Concentration0.5 mg/mL
TargetLy-6G
ImmunogenLy-6G transfected EL-4J cell line.
PurityThe antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

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