Spark Red™ 718 anti-mouse IL-2 Antibody, IL-2, JES6-5H4,BioLegend,503851
ELISA Detection1-3or ELISPOT Detection4-6: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (Cat. Nos. 503701 & 503702) as capture antibody and recombinant mouse IL-2 (Cat. No. 575409) as the standard.Flow Cytometry8-10: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations.Neutralization1,7: The Ultra-LEAF™ purified antibody (Endotoxinin vivoandin vitro(Cat. No. 503845-503850)) is recommended for neutralization.Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections2,in vivocapture7, and immunocytochemistry.Note:For testing mouse IL-2 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. No. 431001 & 431004) are specially developed and recommended.
Host
Rat
Reactivity
Mouse
Application
ICFC - Quality tested
Platform ID
BAB740846276
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameSpark Red™ 718 anti-mouse IL-2 Antibody, IL-2, JES6-5H4
Cat. No.503851
HostRat
RRIDAB_2936721 (BioLegend Cat. No. 503851)AB_2936721 (BioLegend Cat. No. 503852)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberJES6-5H4
Concentration0.5 mg/mL
TargetIL-2
ImmunogenE. coli-expressed, recombinant mouse IL-2
PurityThe antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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