Spark UV™ 387 anti-human HLA-A,B,C Antibody, HLA-A,B,C, W6/32,BioLegend,311462
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC - Quality tested
Platform ID
BAB713137054
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameSpark UV™ 387 anti-human HLA-A,B,C Antibody, HLA-A,B,C, W6/32
Cat. No.311462
HostMouse
RRIDAB_3083303 (BioLegend Cat. No. 311461)AB_3083303 (BioLegend Cat. No. 311462)
IsotypeMouse IgG2a, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberW6/32
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetHLA-A HLA-B HLA-C
PurityThe antibody was purified by affinity chromatography and conjugated with Spark UV™ 387 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+T cells. They play an important role in cell-mediated immune responses and tumor surveillance.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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