Spark Violet™ 538 anti-human HLA-DR Antibody, HLA-DR, L243,BioLegend,307678
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8,in vitroblocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC - Quality tested
Platform ID
BAB744044740
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameSpark Violet™ 538 anti-human HLA-DR Antibody, HLA-DR, L243
Cat. No.307678
HostMouse
RRIDAB_2890742 (BioLegend Cat. No. 307677)AB_2890742 (BioLegend Cat. No. 307678)
IsotypeMouse IgG2a, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberL243
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetHLA-DR
PurityThe antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+T cells.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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