StarBright UltraViolet 740 anti-human IFN-γ antibody, IFN-?, 4S.B3,BioLegend,502561
ELISA or ELISPOT Detection5:The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry3,4,6-8:The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include:neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Application
ICFC - Quality tested
Platform ID
BAB187222028
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameStarBright UltraViolet 740 anti-human IFN-γ antibody, IFN-?, 4S.B3
Cat. No.502561
HostMouse
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone Number4S.B3
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.) The concentration relates to the dye component of the conjugate.
TargetIFN-gamma
ImmunogenPartially purified, native human IFN-γ
PurityThe antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
FormulationPhosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use ourPanel Selector toolto build your ideal panel. For ordering assistance or to request a sample,contact your local field representative.This product is also available for direct purchase within aStarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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