T7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated,Bioss,bsm-33012M-BF680

Host

Mouse

Reactivity

Species independent

Application

WB

Conjugate

AbBy Fluor-680

Platform ID

BAB819562037

Bioss

Headquarters

300 Tradecenter Dr, ste 4610Woburn, Massachusetts 01801 - U.S.A.

Contact

Tel: +1.781.569.5821
Fax: +1.781.731.9286

Product Specifications
Scientific Background
Synonyms

Specifications

NameT7 tag (3G7) Monoclonal Antibody, AbBy Fluor-680 Conjugated
Cat. No.bsm-33012M-BF680
HostMouse
IsotypeIgG
ReactivitySpecies independent
ConjugationAbBy Fluor-680
ApplicationWB
Working DilutionsWB:1:300-5000
ClonalityMonoclonal
Concentration1ug/ul
PurityPurified by Protein A.
FormulationAqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
StorageStore at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Scientific Background

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.

Synonyms

T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag.

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