TotalSeq™-A0322 anti-mouse Hashtag 22 Antibody, H-2/CD45, M1/42; 30-F11,BioLegend,113943

TotalSeq™ anti-mouse Hashtag reagent is a mixture of two monoclonal antibodies conjugated to the same oligonucleotide. The antibodies are specific against mouse CD45 and MHC class I (of a, b,  d, j, k, s, and u haplotypes) and can be used to label hematopoietic and non-hematopoietic cells in most commonly used mouse strains for multiplex single cell sequencing analysis. The conjugated antibodies are pre-mixed to be used following an optimized protocol similar to the CITE-seq workflow.CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. CD45 plays a key role in TCR and BCR signal transduction.MHC class I is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The M1/42 antibody reacts with the H-2 MHC class I alloantigens expressed on nucleated cells from mice of the a, b, d, j, k, s, and u haplotypes (Stallcup, KCet al, 1981).Importantly, some cell lines or experimental models may lack or express very low levels of either or both CD45 and MHC I molecules. We recommend a pilot experiment before single cell proteogenomics to confirm expression of these molecules in your experimental system. Different mouse strains express different MHC I haplotypes. Please refer to thesupplemental tablefor detailed information about the haplotype of your experimental model. The supplemental table and summary below are not exhaustive and provide just a summary of common laboratory mouse strains recognized by TotalSeq™ anti-mouse Hashtags.Mouse strains recognized by clone M1/42:129/-; A/J; AKR/J; BALB/cAnN; BALB/cBy; BALB/CJ; BXSB/Mp; C3H/Bi; C3H/He; C3HeB/FeJ; C57BL/6; C57BL/10; C57BLR/cdj; C57L/J; C58/J; C.B-17; CBA/Ca; CBA/J; CBA/N; CE/J; DA/HuSn; GRS/J; HRS/J; I/LnJ; LP/J; MA/MyJ; MRL/Mp; NOD; NZB/-; PL/J; RF/J; SEC/-; SJL/J; ST/bJ; SWR/J.Strains that are not recognized by clone M1/42: BDP/J; BUB/BnJ; DBA/1; FVB/N; NZW/-; P/J; SM/J.