TotalSeq™-B0073 anti-mousehuman CD44 Antibody, CD44, IM7,BioLegend,103071

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3,in vivoinhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Host

Rat

Reactivity

Mouse, Human

Application

PG -Quality tested

Platform ID

BAB801328495

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameTotalSeq™-B0073 anti-mousehuman CD44 Antibody, CD44, IM7
Cat. No.103071
HostRat
RRIDAB_2832299 (BioLegend Cat. No. 103071)
IsotypeRat IgG2b, κ
ReactivityMouse, Human
ApplicationPG -Quality tested
ClonalityMonoclonal
Clone NumberIM7
Concentration0.5 mg/mL
TargetCD44
ImmunogenDexamethasone-induced myeloid leukemia M1 cells
PurityThe antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

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