TotalSeq™-B0074 anti-mouse CD54 Antibody, CD54, YN1/1.7.4,BioLegend,116139

Additional reported applications (for the relevant formats) include:in vitroandin vivoblocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Host

Rat

Reactivity

Mouse

Application

PG -Quality tested

Platform ID

BAB040847264

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameTotalSeq™-B0074 anti-mouse CD54 Antibody, CD54, YN1/1.7.4
Cat. No.116139
HostRat
RRIDAB_2832397 (BioLegend Cat. No. 116139)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationPG -Quality tested
ClonalityMonoclonal
Clone NumberYN1/1.7.4
Concentration0.5 mg/mL
TargetCD54
ImmunogenMouse NS-1 cells
PurityThe antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

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