TotalSeq™-B0231 anti-mouse H-2Kbbound to SIINFEKL Antibody, H-2Kbbound to SIINFEKL, 25-D1.16,BioLegend,141619

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3, and inhibition of T cell response to H-2Kb-SIINFEKLin vitro.

Host

Mouse

Reactivity

Mouse

Application

PG - Quality tested

Platform ID

BAB736059839

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameTotalSeq™-B0231 anti-mouse H-2Kbbound to SIINFEKL Antibody, H-2Kbbound to SIINFEKL, 25-D1.16
Cat. No.141619
HostMouse
RRIDAB_2941424 (BioLegend Cat. No. 141619)
IsotypeMouse IgG1, κ
ReactivityMouse
ApplicationPG - Quality tested
ClonalityMonoclonal
Clone Number25-D1.16
Concentration0.5 mg/mL
TargetH-2Kb bound to SIINFEKL
ImmunogenSIINFEKL pulsed RMA-S cells
PurityThe antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC)in vivo.

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