TotalSeq™-B1150 anti-human IL-12/IL-23 p40 Antibody, IL-12/IL-23 p40, C11.5,BioLegend,501825

Flow Cytometry3,5:The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization6:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813).Additional reported applications (for the relevant formats) include:Western blotting, ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry.Note:For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.

Host

Mouse

Reactivity

Human

Application

ICPG - Quality tested

Platform ID

BAB088047126

BioLegend

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Contact

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Product Specifications
Scientific Background

Specifications

NameTotalSeq™-B1150 anti-human IL-12/IL-23 p40 Antibody, IL-12/IL-23 p40, C11.5
Cat. No.501825
HostMouse
RRIDAB_2941588 (BioLegend Cat. No. 501825)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationICPG - Quality tested
ClonalityMonoclonal
Clone NumberC11.5
Concentration0.5 mg/mL
TargetIL-12/IL-23 p40
ImmunogenCHO-expressed, recombinant human IL-12 p70 heterod
PurityThe antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.

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