TotalSeq™-C1132 anti-His-Tag antibody, His Tag, J095G46,BioLegend,362627

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.Clone J095G46 has been verified for immunocytochemistry (ICC).

Host

Mouse

Reactivity

Human, Mouse, Rat, All species

Application

PG - Quality tested

Platform ID

BAB682351838

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameTotalSeq™-C1132 anti-His-Tag antibody, His Tag, J095G46
Cat. No.362627
HostMouse
RRIDAB_2892437 (BioLegend Cat. No. 362627)
IsotypeMouse IgG2a, κ
ReactivityHuman, Mouse, Rat, All species
ApplicationPG - Quality tested
ClonalityMonoclonal
Clone NumberJ095G46
Concentration0.5 mg/mL
TargetHis Tag
ImmunogenKLH-conjugated His-tag peptide.
PurityThe antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
StorageThe antibody solution should be stored undiluted between 2°C and 8°C.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.

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