TotalSeq™-C1214 anti-GATA1 Antibody, GATA1, P84F5,BioLegend,939107
This clone was tested for ICC using 4% PFA-fixed HEL cells permeabilized with either methanol or Triton X-100. While both methods were compatible with robust HEL staining, Triton X-100 permabilization resulted in stronger staining.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor GATA1 staining.True-Nuclear™ Transcription Factor Buffer Set allowed for better binding to the epitope when compared to the True Phos perm buffer, there was a twofold difference between their median PE readings.
Host
Mouse
Reactivity
Human
Application
ICPG - Quality tested
Platform ID
BAB890696842
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Specifications
Scientific Background
The GATA family of transcription factors is comprised of six members characterized by their binding to the consensus DNA sequence (T/A)GATA(A/G). GATA1, which was the initially described member of the GATA family, is predominantly expressed in cells of the myeloid lineage such as erythrocytes, eosinophils, mast cells, and megacaryocytes. Over the course of erythroid differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exchanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch. Genes induced by GATA1 includeHBB,HBG1/2,ALAS2andHMBS. Haploinsufficient heterozygousGATA1knockout mice spontaneously develop erythroblastic leukemia, andGATA1-null mice die early in development due to profound anemia.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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