TriMethyl-Histone H3-K27 Mouse mAb- ABclonal,ABclonal,A16199

Reactivity

Human, Mouse, Rat

Application

WB, IHC-P, IF/ICC, DB, ChIP, CUT&Tag, ELISA

Conjugate

Unconjugated

Platform ID

BAB304554417

ABclonal

Headquarters

500W Cummings Park, Ste. 6500 Woburn, MA 01801

ABclonal

Contact

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Fax:

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameTriMethyl-Histone H3-K27 Mouse mAb- ABclonal
Cat. No.A16199
Accession NumberP68431
Gene ID (Entrez)8350
RRIDAB_2763651
IsotypeIgG1,kappa
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
ApplicationWB, IHC-P, IF/ICC, DB, ChIP, CUT&Tag, ELISA
Working DilutionsWB:1:2000 - 1:10000 | IHC-P:1:50 - 1:200 | IF/ICC:1:50 - 1:200 | DB:1:500 - 1:2000 | ChIP:5μg antibody for 5μg-10μg of Chromatin | CUT&Tag:10⁵ cells /1 μg | ELISA:Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Clone NumberAMC0015
Molecular Weight17 kDa
ImmunogenSynthetic peptide. This information is considered to be commercially sensitive.
PurityAffinity purification
Appearance/FormLiquid
StorageStore at -20℃. Avoid freeze / thaw cycles.; Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Regulatory StatusResearch Use Only

Scientific Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Synonyms

H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K27

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