USP9X (D4Y7W) Rabbit Monoclonal Antibody (BSA and Azide Free)#63924,Cell Signaling Technology (CST),63924

Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) respectively (1,2). DUBs are categorized into five subfamilies-USP, UCH, OTU, MJD, and JAMM. Ubiquitin-specific protease 9, X-linked (USP9X) possesses a well-conserved catalytic domain with cysteine peptidase activity, which allows for cleavage of ubiquitin and polyubiquitin conjugates. USP9X is the mammalian homolog of theDrosophila fat-facets(faf) gene, which is essential for normal eye development and viability of the early fly embryo (3,4). While USP9X expression is also critical for normal mammalian development (5-7), many of its substrates are only beginning to be elucidated. There is mounting evidence that USP9X functions in the formation of epithelial cell-cell contacts through deubiquitination-dependent stabilization of molecules involved in maintaining the integrity of both adherens and tight junctions. Indeed, USP9X has been found to associate with AF-6, the β-catenin-E-cadherin complex, and EFA6 (8-11). Research studies have also demonstrated that USP9X is an integral component of the TGF-β/BMP signaling cascade by opposing TRIM33-mediated monoubiquitination of SMAD4 (12). USP9X is overexpressed in a variety of human cancers and contributes to enhanced cell survival, in part, through its ability to deubiquitinate and stabilize the Mcl-1 oncoprotein (13). There is some evidence, however, that suggests the role of USP9X in tumorigenesis is context dependent. Research studies have implicated USP9X in a tumor suppressor role during the early stages of pancreatic ductal adenocarcinoma (PDAC) and in an oncogenic role during advanced stages of PDAC (14,15).Nijman, S.M. et al. (2005)Cell123, 773-86.Nalepa, G. et al. (2006)Nat Rev Drug Discov5, 596-613.Huang, Y. et al. (1995)Science270, 1828-31.Huang, Y. and Fischer-Vize, J.A. (1996)Development122, 3207-16.Pantaleon, M. et al. (2001)Mech Dev109, 151-60.Noma, T. et al. (2002)Mech Dev119 Suppl 1, S91-5.Xu, J. et al. (2005)J Neurosci Res80, 47-55.Taya, S. et al. (1998)J Cell Biol142, 1053-62.Taya, S. et al. (1999)Genes Cells4, 757-67.Murray, R.Z. et al. (2004)Mol Biol Cell15, 1591-9.Théard, D. et al. (2010)EMBO J29, 1499-509.Dupont, S. et al. (2009)Cell136, 123-35.Schwickart, M. et al. (2010)Nature463, 103-7.Pérez-Mancera, P.A. et al. (2012)Nature486, 266-70.Cox, J.L. et al. (2014)Cancer Biol Ther15, 1042-52.Alternate NamesDeubiquitinating enzyme FAF-X; DFFRX; Drosophila fat facets related, X-linked; FAF; FAM; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; hFAM; MRX99; MRXS99F; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; ubiquitin specific peptidase 9 X-linked; ubiquitin specific peptidase 9, X-linked; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; USP9; USP9X

Deubiquitinating enzyme FAF-X; DFFRX; Drosophila fat facets related, X-linked; FAF; FAM; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; hFAM; MRX99; MRXS99F; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; ubiquitin specific peptidase 9 X-linked; ubiquitin specific peptidase 9, X-linked; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; USP9; USP9X