Ultra-LEAF™ Purified anti-DYKDDDDK Epitope Tag Antibody, W22083A,BioLegend,624452
W22083A is not suitable for ICFC application.For ICC, fixation permeabilization with 100% ice-cold methanol, or with 4% PFA followed by 100% ice-cold methanol is recommended at the indicated concentration. Fixation/permeabilization with 4% PFA followed by 0.5% Triton-X results in dimmer staining, with an optimal concentration in the 0.1 - 0.5 µg/mL range.For use of this antibody (clone W22083A) in Western blot, this antibody has been demonstrated to have approximately 25% better sensitivity than commonly used anti-DYKDDDDK clone L5.For use of this antibody (clone W22083A) in ICC, this antibody has increased background staining compared to commonly used anti-DYKDDDDK (clone L5).
Host
Rat
Reactivity
Epitope tag
Application
WB - Quality testedICC, IP - Verified
Platform ID
BAB899281455
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-DYKDDDDK Epitope Tag Antibody, W22083A
Cat. No.624452
HostRat
RRIDAB_3083222 (BioLegend Cat. No. 624451)AB_3083222 (BioLegend Cat. No. 624452)
IsotypeRat IgG2b, κ
ReactivityEpitope tag
ApplicationWB - Quality testedICC, IP - Verified
ClonalityMonoclonal
Clone NumberW22083A
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetEpitope tag
ImmunogenDYKDDDDK peptide conjugated to KLH
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and immunocytochemistry. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
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