Ultra-LEAF™ Purified anti-DYKDDDDK Tag Antibody, DYKDDDDK, L5,BioLegend,637328
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
Host
Rat
Reactivity
Epitope tag
Application
WB -Quality testedIP, ICC, ELISA, FC, Purification -Reported in the literature, not verified in house
Platform ID
BAB994569488
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-DYKDDDDK Tag Antibody, DYKDDDDK, L5
Cat. No.637328
HostRat
RRIDAB_2810691 (BioLegend Cat. No. 637327)AB_2810692 (BioLegend Cat. No. 637328)
IsotypeRat IgG2a, λ
ReactivityEpitope tag
ApplicationWB -Quality testedIP, ICC, ELISA, FC, Purification -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberL5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetDYKDDDDK
ImmunogenDYKDDDDK-tagged mouse Langerin
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
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