Ultra-LEAF Purified anti-HA.11 Epitope Tag Antibody anti-HA.11 - 16B12,BioLegend,901521
Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
Host
Mouse
Application
WB - Quality testedFC - VerifiedPurification - Reported in the literature, not verified in house
Platform ID
BAB918583450
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-HA.11 Epitope Tag Antibody anti-HA.11 - 16B12
Cat. No.901521
HostMouse
RRIDAB_2716058 (BioLegend Cat. No. 901521)AB_2716059 (BioLegend Cat. No. 901522)
IsotypeMouse IgG1, κ
ApplicationWB - Quality testedFC - VerifiedPurification - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number16B12
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetHA.11
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
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