Ultra-LEAF Purified anti-His Tag Antibody, His Tag, J095G46,BioLegend,362615
This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.Clone J095G46 has been verified for immunocytochemistry (ICC).
Host
Mouse
Reactivity
Human, Mouse, Rat, All species
Application
WB -Quality testedICC, FC -Verified
Platform ID
BAB307364332
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-His Tag Antibody, His Tag, J095G46
Cat. No.362615
HostMouse
RRIDAB_2814310 (BioLegend Cat. No. 362615)AB_2814311 (BioLegend Cat. No. 362616)
IsotypeMouse IgG2a, κ
ReactivityHuman, Mouse, Rat, All species
ApplicationWB -Quality testedICC, FC -Verified
ClonalityMonoclonal
Clone NumberJ095G46
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetHis Tag
ImmunogenKLH-conjugated His-tag peptide.
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.
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