Ultra-LEAF Purified anti-beta Catenin 1 CTNNB1 Antibody anti-beta Catenin 1 - 12F7,BioLegend,844603

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy4and immunohistochemical staining3.Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Host

Mouse

Reactivity

Human, Mouse, Rat

Application

WB -Quality tested

Platform ID

BAB039570406

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-beta Catenin 1 CTNNB1 Antibody anti-beta Catenin 1 - 12F7
Cat. No.844603
HostMouse
RRIDAB_2565805 (BioLegend Cat. No. 844603)
IsotypeMouse IgG1, κ
ReactivityHuman, Mouse, Rat
ApplicationWB -Quality tested
ClonalityMonoclonal
Clone Number12F7
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
Targetbeta Catenin 1
ImmunogenRecombinant β-catenin (N-terminal fragment) fused to MBP.
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

β-catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β-catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. Finally, this protein binds to the product of the adenomatous polyposis coli (APC) gene, which is mutated in the adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer.

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