Ultra-LEAF™ Purified anti-human B7-H6 Antibody, B7-H6, W20205G,BioLegend,605954

B7-H6 is a type I transmembrane protein and member of the B7 family. It contains 454 amino acids with a predicted molecular weight of 51 kD and has several potential N-glycosylation sites. Similar to the other members of the B7 family, B7-H6 possesses two Ig like domains in the extracellular domain. The most related proteins to B7-H6 are B7-H1 and B7-H3. B7-H6 is expressed in human cancer cells such as myeloid and lymphoblastic leukemia, neuroblastomas, and melanomas, and it binds to NKp30 to activate human NK cells and target cell killing. Nevertheless, tumor cells can escape NK-cell-mediated recognition. A mechanism of immune escape involves tumor cell shedding of B7-H6. That process is mediated by ADAM10 and ADAM17. Thus, soluble B7-H6 high levels in blood have been detected in patients with malignant melanoma; levels that correlate with elevated B7-H6 expression in melanoma biopsies. In addition, B7-H6 is expressed in glioma stem-like cells derived from glioma cell lines in vitro. Soluble isoforms form of B7-H6 have also been detected in serum during normal pregnancy. These isoforms, of ∼37kD and ∼30kD, maintain the C- and N-terminus, and it seems that they are released by a mechanism different from proteolytic cleavage. B7-H6 can be induced in healthy cells by inflammatory process. B7-H6 and NKp30 orthologues do not exist in mice.