Ultra-LEAF™ Purified anti-human C3a/C3a(desArg)/C3 Antibody, C3a/C3a(desArg)/C3, K13/16,BioLegend,518105

The K13/16 antibody reacts with human C3a, C3a(desArg) and C3. The K13/16 antibody can neutralize the bioactivity of natural or recombinant C3a.ELISA Capture:The purified K13/16 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated D17/1 antibody (Cat. No. 518002) as the detecting antibody.Neutralization:The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human C3a/C3a(desArg)/C3 bioactivity.

Host

Mouse

Reactivity

Human

Application

ELISA Capture -Quality testedIP, WB, Neut -Reported in the literature, not verified in house

Platform ID

BAB804227103

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-human C3a/C3a(desArg)/C3 Antibody, C3a/C3a(desArg)/C3, K13/16
Cat. No.518105
HostMouse
RRIDAB_2861044 (BioLegend Cat. No. 518105)AB_2861045 (BioLegend Cat. No. 518106)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationELISA Capture -Quality testedIP, WB, Neut -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberK13/16
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetC3a/C3adesArg/C3
ImmunogenHuman C3a
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

C3, complement component 3, is a 195 kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.

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