Ultra-LEAF™ Purified anti-human CD117 (c-kit) Antibody, CD117, A3C6E2,BioLegend,323418

Additional reported applications (for the relevant formats) include: Blocking the binding of SCF. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 323417 & 323418).

Host

Mouse

Reactivity

Human

Application

FC - Quality testedBlock - Reported in the literature

Platform ID

BAB765345775

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-human CD117 (c-kit) Antibody, CD117, A3C6E2
Cat. No.323418
HostMouse
RRIDAB_2888744 (BioLegend Cat. No. 323417)AB_2888744 (BioLegend Cat. No. 323418)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC - Quality testedBlock - Reported in the literature
ClonalityMonoclonal
Clone NumberA3C6E2
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD117
ImmunogenMOLM-1 megakaryocitic cell line
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemic cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117, and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells. The A3C6E2 antibody potently blocks the binding of SCF.

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