Ultra-LEAF Purified anti-human CD11a Antibody, CD11a, HI111,BioLegend,301234
Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301233 & 301234).
Host
Mouse
Reactivity
Human
Application
FC -Quality testedIHC-F, WB, Block -Reported in the literature, not verified in house
Platform ID
BAB855913546
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human CD11a Antibody, CD11a, HI111
Cat. No.301234
HostMouse
RRIDAB_2832576 (BioLegend Cat. No. 301233)AB_2832577 (BioLegend Cat. No. 301234)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality testedIHC-F, WB, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberHI111
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD11a
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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