Ultra-LEAF™ Purified anti-human CD16 Antibody, CD16, W18182B,BioLegend,310010

Clone W18182B can completely block the binding of clone B73.1 on target cells.Clone W18182B does not affect the binding of clones 3G8 or W18182A on target cells.Clone W18182B is able to activate NK cells and induce degranulation.

Host

Rat

Reactivity

Human

Application

FC - Quality testedActiv - Verified

Platform ID

BAB644158915

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-human CD16 Antibody, CD16, W18182B
Cat. No.310010
HostRat
RRIDAB_3720508 (BioLegend Cat. No. 310010)AB_3720508 (BioLegend Cat. No. 310011)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationFC - Quality testedActiv - Verified
ClonalityMonoclonal
Clone NumberW18182B
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD16
ImmunogenRecombinant human CD16 protein
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

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