Ultra-LEAF Purified anti-human CD210 IL-10 R Antibody, CD210, 3F9,BioLegend,308817

Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8Additional reported applications (for the relevant formats) include: immunoprecipitation1,in vitroblocking1-3of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).

Host

Rat

Reactivity

Human, Cynomolgus, Rhesus

Application

FC -Quality testedIP, Block -Reported in the literature, not verified in house

Platform ID

BAB933598768

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-human CD210 IL-10 R Antibody, CD210, 3F9
Cat. No.308817
HostRat
RRIDAB_2800804 (BioLegend Cat. No. 308817)AB_2800805 (BioLegend Cat. No. 308818)
IsotypeRat IgG2a, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC -Quality testedIP, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number3F9
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD210
ImmunogenshIL-10R
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

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