Ultra-LEAF Purified anti-human CD3 Antibody anti-CD3 - HIT3a,BioLegend,300332

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Host

Mouse

Reactivity

Human

Application

FC -Quality tested

Platform ID

BAB859211962

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-human CD3 Antibody anti-CD3 - HIT3a
Cat. No.300332
HostMouse
RRIDAB_11147368 (BioLegend Cat. No. 300331)AB_11150396 (BioLegend Cat. No. 300332)AB_2616669 (BioLegend Cat. No. 300333)AB_2616670 (BioLegend Cat. No. 300334)
IsotypeMouse IgG2a, κ
ReactivityHuman
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberHIT3a
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD3
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

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