Ultra-LEAF Purified anti-human CD3 Antibody anti-CD3 - UCHT1,BioLegend,300466
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Mouse
Reactivity
Human
Application
FC -Quality testedCyTOF®- VerifiedIHC-F, IP, Activ, WB -Reported in the literature, not verified in house
Platform ID
BAB161911654
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human CD3 Antibody anti-CD3 - UCHT1
Cat. No.300466
HostMouse
RRIDAB_11147760 (BioLegend Cat. No. 300437)AB_11146991 (BioLegend Cat. No. 300438)AB_2616677 (BioLegend Cat. No. 300465)AB_2616678 (BioLegend Cat. No. 300466)AB_2749887 (BioLegend Cat. No. 300473)AB_2749892 (BioLegend Cat. No. 300474)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality testedCyTOF®- VerifiedIHC-F, IP, Activ, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberUCHT1
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD3
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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