Ultra-LEAF Purified anti-human CD31 Antibody, CD31, WM59,BioLegend,303144
Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC -Quality testedIHC, ICC, Block -Reported in the literature, not verified in house
Platform ID
BAB602179075
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human CD31 Antibody, CD31, WM59
Cat. No.303144
HostMouse
RRIDAB_2819927 (BioLegend Cat. No. 303143)AB_2819928 (BioLegend Cat. No. 303144)
IsotypeMouse IgG1, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC -Quality testedIHC, ICC, Block -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberWM59
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD31
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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