Ultra-LEAF™ Purified anti-human CD4 Antibody, CD4, OKT4,BioLegend,317454
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC -Quality testedIHC-F -Reported in the literature, not verified in house
Platform ID
BAB811212484
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-human CD4 Antibody, CD4, OKT4
Cat. No.317454
HostMouse
RRIDAB_2876620 (BioLegend Cat. No. 317453)AB_2876620 (BioLegend Cat. No. 317454)
IsotypeMouse IgG2b, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC -Quality testedIHC-F -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberOKT4
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD4
ImmunogenHuman peripheral T cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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