Ultra-LEAF Purified anti-human CD4 Antibody, CD4, RPA-T4,BioLegend,300570
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
Host
Mouse
Reactivity
Human
Application
FC -Quality testedCyTOF® -VerifiedActiv, Block, IHC -Reported in the literature, not verified in house
Platform ID
BAB025054848
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human CD4 Antibody, CD4, RPA-T4
Cat. No.300570
HostMouse
RRIDAB_2810426 (BioLegend Cat. No. 300569)AB_2810427 (BioLegend Cat. No. 300570)AB_2810428 (BioLegend Cat. No. 300571)AB_2810429 (BioLegend Cat. No. 300572)AB_2810430 (BioLegend Cat. No. 300573)AB_2810431 (BioLegend Cat. No. 300574)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality testedCyTOF® -VerifiedActiv, Block, IHC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRPA-T4
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD4
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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