Ultra-LEAF Purified anti-human HLA-A,B,C Antibody anti-HLA-A,B,C - W6/32,BioLegend,311442
Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Host
Mouse
Reactivity
Human, Cynomolgus, Rhesus
Application
FC -Quality testedActiv, Block, IHC-F, IP, WB -Reported in the literature, not verified in house
Platform ID
BAB463112066
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human HLA-A,B,C Antibody anti-HLA-A,B,C - W6/32
Cat. No.311442
HostMouse
RRIDAB_2783175 (BioLegend Cat. No. 311427)AB_2561492 (BioLegend Cat. No. 311428)AB_2800814 (BioLegend Cat. No. 311441)AB_2800815 (BioLegend Cat. No. 311442)AB_2783176 (BioLegend Cat. No. 311447)AB_2783177 (BioLegend Cat. No. 311448)
IsotypeMouse IgG2a, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC -Quality testedActiv, Block, IHC-F, IP, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberW6/32
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetHLA-A
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>IHC Antibodies
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