Ultra-LEAF™ Purified anti-human IFN-γ Antibody, IFN-γ, MD-1,BioLegend,507503

ELISA1,2,5and ELISPOT3,4Capture:The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended.Neutralization1:The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513).Additional reported applications (for the relevant formats) include:Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7of paraformaldehyde-fixed, saponin-treated frozen tissue sections.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Host

Mouse

Reactivity

Human

Application

ELISA Capture -Quality testedICFC, IHC-F, WB -Reported in the literature, not verified in house

Platform ID

BAB777717305

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-human IFN-γ Antibody, IFN-γ, MD-1
Cat. No.507503
HostMouse
RRIDAB_2810641 (BioLegend Cat. No. 507503)AB_2810642 (BioLegend Cat. No. 507504)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationELISA Capture -Quality testedICFC, IHC-F, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMD-1
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIFN-gamma
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 4S.B3 antibody reacts with the human IFN-γ. The MD-1 antibody reacts with human and monkey interferon-gamma (IFN-γ). The MD-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

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