Ultra-LEAF™ Purified anti-human IL-1α Antibody, IL-1α, 364-3B3-14,BioLegend,500109
ELISA or ELISPOT Capture1:The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.Flow Cytometry4:The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Additional reported applications (for the relevant formats) include:immunohistochemistry3. The 364-3B3-14 is a non-neutralizing antibody.
Host
Mouse
Reactivity
Human
Application
ELISA Capture - Quality testedELISPOT Capture, ICFC, IHC - Reported in the literature, not verified in house
Platform ID
BAB556212414
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-human IL-1α Antibody, IL-1α, 364-3B3-14
Cat. No.500109
HostMouse
RRIDAB_2890851 (BioLegend Cat. No. 500108)AB_2890851 (BioLegend Cat. No. 500109)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationELISA Capture - Quality testedELISPOT Capture, ICFC, IHC - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number364-3B3-14
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-1alpha
ImmunogenRecombinant human IL-1α
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The 364-3B3-14 antibody is a non-neutralizing antibody.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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