Ultra-LEAF™ Purified anti-human IL-12/IL-23 p40 Antibody, IL-12/IL-23 (p40), C11.5,BioLegend,501824
Flow Cytometry3,5:The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization6:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813).Additional reported applications (for the relevant formats) include:Western blotting, ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry.Note:For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Application
ICFC -Quality testedIHC-F, ICC, Block, Neut, WB -Reported in the literature, not verified in house
Platform ID
BAB338072114
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-human IL-12/IL-23 p40 Antibody, IL-12/IL-23 (p40), C11.5
Cat. No.501824
HostMouse
RRIDAB_2861030 (BioLegend Cat. No. 501823)AB_2861031 (BioLegend Cat. No. 501824)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationICFC -Quality testedIHC-F, ICC, Block, Neut, WB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberC11.5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-12/IL-23 p40
ImmunogenCHO-expressed, recombinant human IL-12 p70 heterod
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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