Ultra-LEAF™ Purified anti-human IL-2 Antibody, IL-2, MQ1-17H12,BioLegend,500354
ELISA or ELISPOT Capture2,3:The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include:immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.Note:For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat15
Host
Rat
Reactivity
Human
Application
ELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, ICFC, IHC, IP -Reported in the literature, not verified in house
Platform ID
BAB819819615
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-human IL-2 Antibody, IL-2, MQ1-17H12
Cat. No.500354
HostRat
RRIDAB_2861026 (BioLegend Cat. No. 500353)AB_2861027 (BioLegend Cat. No. 500354)
IsotypeRat IgG2a, κ
ReactivityHuman
ApplicationELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, ICFC, IHC, IP -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMQ1-17H12
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-2
ImmunogenE. coli- expressed recombinant human IL-2
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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