Ultra-LEAF Purified anti-human IL-3 Antibody anti-IL-3 - BVD8-3G11,BioLegend,500506
ELISA1-4or ELISPOT Capture:The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.Additional reported applications include:Western blotting4and immunoprecipitation4.
Host
Rat
Reactivity
Human
Application
Block - Quality testedIP, WB - Reported in the literature, not verified in house
Platform ID
BAB400053613
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human IL-3 Antibody anti-IL-3 - BVD8-3G11
Cat. No.500506
HostRat
RRIDAB_2616770 (BioLegend Cat. No. 500506)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationBlock - Quality testedIP, WB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberBVD8-3G11
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-3
ImmunogenYeast-expressed, recombinant human IL-3
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.
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