Ultra-LEAF™ Purified anti-human IL-4 Antibody, IL-4, MP4-25D2,BioLegend,500840

ELISA Capture or ELISpot Capture: The MP4-25D2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay.Flow Cytometry6,9:The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.Neutralization1-3:The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Host

Rat

Reactivity

Human

Application

ELISA Capture - Quality testedCyTOF® - VerifiedNeut, ICFC, IHC - Reported in the literature, not verified in house

Platform ID

BAB944082469

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-human IL-4 Antibody, IL-4, MP4-25D2
Cat. No.500840
HostRat
RRIDAB_2810615 (BioLegend Cat. No. 500837)AB_2810616 (BioLegend Cat. No. 500838)AB_2810617 (BioLegend Cat. No. 500839)AB_2810618 (BioLegend Cat. No. 500840)AB_2810619 (BioLegend Cat. No. 500841)AB_2810620 (BioLegend Cat. No. 500842)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationELISA Capture - Quality testedCyTOF® - VerifiedNeut, ICFC, IHC - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMP4-25D2
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-4
ImmunogenCHO-expressed, recombinant human IL-4
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

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