Ultra-LEAF Purified anti-human IL-6 Antibody, IL-6, MQ2-13A5,BioLegend,501125
ELISA or ELISPOT Capture1-3,7:The purified MQ2-13A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MQ2-39C3 antibody (Cat. No. 501202) as the detecting antibody.Neutralization1-3,5,6:The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-6 bioactivity (Cat. No. 501110). The MQ2-13A5 antibody can neutralize the bioactivity of natural or recombinant IL-6.Additional applications (for the relevant formats) include:intracellular flow cytometry10.
Host
Rat
Reactivity
Human
Application
ELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, Neut, ICFC -Reported in the literature, not verified in house
Platform ID
BAB007563248
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
Email:
Product Specifications
Scientific Background
Specifications
NameUltra-LEAF Purified anti-human IL-6 Antibody, IL-6, MQ2-13A5
Cat. No.501125
HostRat
RRIDAB_2810625 (BioLegend Cat. No. 501125)AB_2810626 (BioLegend Cat. No. 501126)
IsotypeRat IgG1, κ
ReactivityHuman
ApplicationELISA Capture -Quality testedCyTOF® -VerifiedELISPOT Capture, Neut, ICFC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMQ2-13A5
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetIL-6
ImmunogenCOS-7- expressed, recombinant human IL-6
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
Request a product
Please provide the required information below so that we can quickly source your products.