Ultra-LEAF Purified anti-mouse CD106 Antibody, CD106, 429 (MVCAM.A),BioLegend,105728

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7of acetone-fixed frozen sections, blocking4,5,8of ligand bindingin vitroandin vivo, immunoprecipitation1, and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).

Host

Rat

Reactivity

Mouse

Application

FC -Quality testedIHC-F, IP -Reported in the literature, not verified in house

Platform ID

BAB497129523

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameUltra-LEAF Purified anti-mouse CD106 Antibody, CD106, 429 (MVCAM.A)
Cat. No.105728
HostRat
RRIDAB_2819809 (BioLegend Cat. No. 105727)AB_2819810 (BioLegend Cat. No. 105728)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedIHC-F, IP -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number429 (MVCAM.A)
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD106
ImmunogenMouse preadipose cell line PA6
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1integrin) and LPAM-1 (α4/β7integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.