Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1R) Antibody, CD115, AFS98,BioLegend,135542
Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
Host
Rat
Reactivity
Mouse
Application
FC -Quality testedBlock -Reported in the literature, not verified in house
Platform ID
BAB017369803
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameUltra-LEAF™ Purified anti-mouse CD115 (CSF-1R) Antibody, CD115, AFS98
Cat. No.135542
HostRat
RRIDAB_2832481 (BioLegend Cat. No. 135537)AB_2832482 (BioLegend Cat. No. 135538)AB_2832483 (BioLegend Cat. No. 135539)AB_2832484 (BioLegend Cat. No. 135540)AB_2832485 (BioLegend Cat. No. 135541)AB_2832486 (BioLegend Cat. No. 135542)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality testedBlock -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberAFS98
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD115
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only
Scientific Background
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Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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