Ultra-LEAF™ Purified anti-mouse CD122 (IL-2Rβ) Antibody, CD122, TM-β1,BioLegend,123223

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking of IL-2 bindingin vivoandin vitro(Cat. No. 123204).

Host

Rat

Reactivity

Mouse

Application

FC -Quality testedIP, Block, Depletion -Reported in the literature, not verified in house

Platform ID

BAB532501272

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse CD122 (IL-2Rβ) Antibody, CD122, TM-β1
Cat. No.123223
HostRat
RRIDAB_2810372 (BioLegend Cat. No. 123223)AB_2810373 (BioLegend Cat. No. 123224)AB_2810374 (BioLegend Cat. No. 123225)AB_2810375 (BioLegend Cat. No. 123226)AB_2810376 (BioLegend Cat. No. 123227)AB_2810377 (BioLegend Cat. No. 123228)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality testedIP, Block, Depletion -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberTM-β1
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD122
ImmunogenRat T cell line expressing mouse CD122 ( IL-2Rβ)
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γcsubunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The TM-β1 antibody does inhibit IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

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