Ultra-LEAF™ Purified anti-mouse CD126 (IL-6Rα chain) Antibody, CD126, D7715A7,BioLegend,115815

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5,in vivoreceptor blocking1-2,4-6,8, andin vitroneutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115808).Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined tonotreact with rat.

Host

Rat

Reactivity

Mouse

Application

FC -Quality testedBlock, IP, Neut -Reported in the literature, not verified in house

Platform ID

BAB155326081

BioLegend

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Contact

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Product Specifications
Scientific Background

Specifications

NameUltra-LEAF™ Purified anti-mouse CD126 (IL-6Rα chain) Antibody, CD126, D7715A7
Cat. No.115815
HostRat
RRIDAB_2810349 (BioLegend Cat. No. 115815)AB_2810350 (BioLegend Cat. No. 115816)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality testedBlock, IP, Neut -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberD7715A7
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.
TargetCD126
ImmunogenOKT4 hybridoma cells
PurityThe Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

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